Liquid Culture Recipe

Use this recipe to create potent and effective liquid cultures to help spur the development of your tasty mushrooms.

By Willoughby Arevalo
Published on August 14, 2019
article image
by Adobestock/MrPreecha

Most LC recipes are formulated to have a sugar content of 4 percent or less by weight. More than this is toxic to mycelium. Other ingredients can be added in small amounts for protein, lipids, starches, minerals, vitamins, and more. Adding a little bit of an infusion or a few particles of the final substrate can speed myceliation during spawn run. Beware that some ingredients cause cloudiness that can disguise contamination. For general purpose, I prefer regular-mouth pint (500 ml) Mason jars. To keep things interesting for your cultures, change up the recipe when expanding them.

You’ll need: a gram scale or measuring spoons, measuring cup, jars, airport lids and rings, agitators (marbles, pieces of broken glass, crystals, or magnetic stir bars), big pot, jar funnel, ladle, paper towel or clean cloth, big spoon or whisk, aluminum foil, and a pressure canner (PC) or alternative.

  1. Measure out all ingredients. Calculate total volume based on how many jars you need/can fit in your PC. Consider saving room for a plain water jar.
  2. Heat water to warm (not boiling) in a pot.
  3. Stir in other ingredients until fully dissolved.
Comments (0) Join others in the discussion!
    Online Store Logo
    Need Help? Call 1-800-234-3368