Liquid Culture Recipe

Use this recipe to create potent and effective liquid cultures to help spur the development of your tasty mushrooms.

Getty Images / ossyugioh

Most LC recipes are formulated to have a sugar content of 4% or less by weight. More than this is toxic to mycelium. Other ingredients can be added in small amounts for protein, lipids, starches, minerals, vitamins, and more. Adding a little bit of an infusion or a few particles of the final substrate can speed myceliation during spawn run. Beware that some ingredients cause cloudiness that can disguise contamination. For general purpose, I prefer regular-mouth pint (500 ml) Mason jars. To keep things interesting for your cultures, change up the recipe when expanding them.

You’ll need: a gram scale or measuring spoons, measuring cup, jars, airport lids and rings, agitators (marbles, pieces of broken glass, crystals, or magnetic stir bars), big pot, jar funnel, ladle, paper towel or clean cloth, big spoon or whisk, aluminum foil, and a pressure canner (PC) or alternative.

  1. Measure out all ingredients. Calculate total volume based on how many jars you need/can fit in your PC. Consider saving room for a plain water jar.
  2. Heat water to warm (not boiling) in a pot.
  3. Stir in other ingredients until fully dissolved.
  4. Using a jar funnel and a measured ladle or pitcher, fill jars not more than halfway.
  5. Drop a stir bar or other agitator into each jar.
  6. Wipe up any drips from the rim of the jars.
  7.  Put on airport lids and screw down rings until snug. Cover jar lids with foil to keep filters dry, and load into PC. Include any syringes or other tools in need of sterilization, wrapped in foil.
  8. Sterilize for 15–20 minutes at 15 psi. Longer cooking will caramelize and toxify the sugars and melt the syringes.

Tissue Culturing to Liquid Culture

Taking biopsy of tissue by stabbing mushroom. - Isabelle Kirouac

This biopsy technique works well with most fleshy mushrooms, though tough or tiny mushrooms can be nearly impossible to catch This way. Waterlogged mushrooms should be allowed to dry out a little in the fridge first. Dry mushrooms can be soaked in 3% hydrogen peroxide to rehydrate. For improved chances, repeat process with multiple jars of media.

You’ll need: a mushroom, a sterile syringe fitted with a 16-gauge needle, a sterilized jar of water with an airport lid, a jar of sterilized LC medium with an airport lid, alcohol in spray bottle, alcohol prep pads, cotton balls, tweezers or knife (optional), permanent marker.

Sasquatch AssScractch
12/21/2020 7:37:06 PM

I've been successfully growing mushrooms for over 20 years. These instructions are NONSENSE, and cannot possibly lead to success... Read Paul Stamets. Forget this simple minded bullshit.

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